Composite

Part:BBa_K3722011:Design

Designed by: Yongzhu Li   Group: iGEM21_NWU-CHINA-A   (2021-09-30)


T7_Promoter-RBS-His Tag-MaFMO-RBS-His Tag-TnaA-T7_Terminator


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 2940
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

Since our team uses In-Fusion technology for seamless cloning, the design of primers in the experiment is very important.


Source

Assembly

References